Josher Lo

Hello! 👋

My name isJOSHER

I am a

About me.

I am Lo Sze Ee, Josher, a 19 year old boy from Singapore. I am currently serving in the Singaporean army, and will resume my studies in 2027. I enjoy programming, learning sciences and playing/listening to music.

Tools I am using now:

GoLang

Raspberry Pi

Svelte

TypeScript

My education history.

2031

2027

University of Cambridge (Clare College)

About this universityThe University of Cambridge is one of the world's best universities. Clare College is the second oldest college in Cambridge. I was offered a place to study at Clare College in the 2025 admissions cycle.

2024

2019

NUS High School of Mathematics and Science

About this schoolNUS High School (NUSH) is a school in Singapore offering a 6-year program, specialising in mathematics and science.

My experiences.

Optimising In Vitro Generation and Characterisation of Stem Cell Derived Photoreceptor Cells For Retinal Therapeutics

Institute of Molecular and Cell Biology (IMCB)

Student ResearcherJanuary 2023 ― December 2023

What is this?This is a group research project on the differentiation protocol of human IPSCs into photoreceptor cells.What did I do?My team and I optimised the differentiation protocol and achieved significant results. This project was lab-based and we carried out some of the procedures with the guidance of our mentor.What did I learn?Lab skills (biological), hIPSCs, differentiation protocols

,Retinal degenerative diseases are one of the leading causes of permanent incurable vision loss and are often characterised by the loss of light-sensing photoreceptors. In vitro differentiation of induced pluripotent stem cells (iPSCs) into photoreceptors is a promising avenue to generate transplantable photoreceptors for vision restoration. Herein, we report an optimised protocol with significant improvements over current research in producing retinal progenitor cells of higher yield and quality. We have shortlisted three factors - seeding density, picking method (needle/pipette picked) together with clump size, and timing of rosette picking which can potentially improve the differentiation process, and this study focuses on their first-time application to retinal cells. We found that higher seeding densities (2M v. 750K) on Day 7 resulted in significantly faster rosette formation – 25% faster than currently reported. Needle-picked rosettes seeded in clumps had more uniformly shaped cells and were more homogenous than those which were pipette picked. Picking rosettes later in the differentiation protocol (Day 18 v. Day 20) reduced the cell detachment rate. Immunostaining, qRT-PCR and brightfield microscopy were employed throughout this study to quantify the quality of cells. The results contribute valuable insights for advancing iPSC-based retinal cell replacement therapies, offering hope for improved treatment outcomes for retinal degenerative disease patients.

Some of my achievements.

International Chemistry Olympiad

SilverJuly 2024

What is this?The International Chemistry Olympiad (IChO), is an annual competition for the world’s most talented chemistry students at the secondary school level. Nations around the world send a team of four students who are tested on their chemistry knowledge and skills in a five-hour laboratory practical exam and a five-hour written theoretical examination that are held on separate days with the practical examination usually being before the theoretical examination.What did I achieve?I achieved a silver medal, and placed 46th out of 330 participants from all over the globe.

Singapore Science and Engineering Fair

SilverMarch 2024

What is this?Singapore Scienad and Engineering Fair (SSEF), is Singapore's national science fair organised by the Ministry of Education (MOE), Agency for Science, Technology and Research (A*STAR) and Science Centre Singapore (SCS).What did my team achieve?We were selected for the finals and obtained a silver medal.

International Chemistry Tournament

Best Reporter, Best Solution, Personal Bronze, Team SilverAugust 2023

What is this?International Chemistry Tournament (IChTo), is an annual chemistry tournament for high school students. Students work in a team of 6, solving 12 open-ended chemistry problems, with no set solution, and requires students to display their knowledge in chemistry and creative minds. Each round consists of the reporting team presenting their solution, the opposition team coming up with flaws in the report, a debate between the reporter and the opposer, and lastly the reviewing team reviewing the entire round. This competition also focuses on a lot on teamwork skills.What did my team achieve?Our team achieved second place. I achieved the best reporter, best solution for a problem and a personal bronze medal. My team achieved 2 more best solutions for problems and 2 more personal silver medals.

SYF Song Writing Competition

Consolation PrizeApril 2018

What is this?This is a Song Writing competition organised by Singapore Youth Festival (SYF). SYF Youth Station is the SYF Celebrations strand started in 2016 dedicated to showcasing students' original musical works.What did I achieve?My partner and I achieved consolation prize, and made it to the top 16. We performed our song, "We are The Youths" live, and had it professional recorded.

My projects.

BOUNDARY

ExpressPythonHTML5NodeJS

An app to determine if a postal code is in a boundary, for hospitals use.

EXPLODING KITTENS

FlutterFirebase

A fun, free online implementation of the fun card game with the same name!